Third Generation RNA Kit for Cellular Reprogramming of Fibroblasts, Blood, and Urine
The latest evolution in Stemgent RNA reprogramming, the StemRNA 3rd Gen Reprogramming Kit (formerly called the StemRNA-NM Reprogramming Kit), combines non-modified RNA and microRNA technology to provide a kit for stem cell researchers with a new level of versatility, simplicity and time savings, enabling cellular reprogramming of human fibroblasts, and cells from blood and now urine for difficult to reprogram patient samples.
Key Benefits
- Flexible technology generates high-quality human iPS cell lines from multiple target cell typesOut-of-the-box reprogramming of cells from skin (fibroblasts), blood (endothelial progenitor cells; EPCs) and urine (urine-derived progenitor cells; UPCs)
- High efficiency, non-integrating reprogrammingStemRNA-3rd Gen requires a few as four additional reagents. Double stranded microRNAs enhance reprogramming efficiency, providing iPS cells with high efficiency (up to 4% from fibroblasts, up to 3% from EPCs, up to 0.5% from UPCs).
- Time-saving protocol delivers faster results facilitating higher throughputColonies ready to pick in 10-14 days from fibroblasts and 12-16 days from EPCs or UPCs. No screening needed
| StemRNA-3rd Gen Reprogramming Kit (00-0076) | |||
|---|---|---|---|
| Feature | Fibroblasts | Urine (UPCs) | Blood (EPCs) |
| No. wells per kit | 9 | 3 | 3 |
| No Transfections required | 4 | 6-8 | 6-8 |
| Days to primary iPS cell colonies | 10-14 | 12-14 | 12-14 |
| Reprogramming efficiency | 2-+4% | 0.1-0.5% | 0.4-3% |
| Screening Required | No | No | No |
| Xeno-compatible protocol | Yes | No | No |
| GMP-compatible RNA manufacturing protocol | Yes | Yes | Yes |
P7 primary iPS cells derived from UPCs were stained using the StainAlive TRA-1-60 Antibody (Cat. No. 09‑0068) and DAPI after expansion in NutriStem hPSC XF Medium (Cat. No. 01‑0005)
iPS cells derived from fibroblasts using the StemRNA-3rd Gen Reprogramming Kit were cultured on iMatrix-511 (Cat. No. NP892‑011) in NutriStem hPSC XF Medium(Cat. No. 01‑0005)for 7 passages. Magnification: 4x
Timeline for StemRNA 3rd reprogramming of fibroblasts
Download our StemRNA-3rd Gen Reprogramming Posters:
ISSCR 2015
ISSCR 2016
